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Redesigned Duplex RT-qPCR for the Detection of GI and GII Human Noroviruses Article

Danlei Liu, Zilei Zhang, Qingping Wu, Peng Tian, Haoran Geng, Ting Xu, Dapeng Wang

Engineering 2020, Volume 6, Issue 4,   Pages 442-448 doi: 10.1016/j.eng.2019.08.018

Abstract: ) and reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR).The widely used primers and probes for RT-qPCR were established in the early 2000s.In this study, a new duplex RT-qPCR (ND-RT-qPCR) was designed for the detection of genogroup RT-qPCR (Kageyama RT-qPCR) for 23 HuNoV-positive clinical samples.The sensitivity reflected by the Cq value was lower in ND-RT-qPCR than in Kageyama RT-qPCR.

Keywords： Human noroviruses     RT-qPCR     Redesign     Primer     Probe     Detection    

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quantification of 3′-terminal 2′-O-methylated small RNAs by utilizing oxidative deep sequencing and stem-loop RT-qPCR

Frontiers of Medicine 2022, Volume 16, Issue 2,   Pages 240-250 doi: 10.1007/s11684-021-0909-7

Abstract: We further compared the efficiency of stem-loop and poly(A)-tailed RT-qPCR in quantifying plant miRNAsin animal tissues and identified stem-loop RT-qPCR as the only suitable approach.Likewise, stem-loop RT-qPCR was superior to poly(A)-tailed RT-qPCR in quantifying 3′-terminal 2′-O-methyl

Keywords： small RNAs     2′-O-methylation     sequencing     RT-qPCR    

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Developing a Transformation-Independent and Unbiased qPCR Assay to Rapidly Evaluate the Determinants Article

Xiaoyan Ma, Xinxin Liang, Yi-Xin Huo

Engineering 2019, Volume 5, Issue 4,   Pages 803-810 doi: 10.1016/j.eng.2019.06.002

Abstract: transformation-independent method was established using a modified quantitative polymerase chain reaction (qPCRThis qPCR-based measurement was tested by the commonly used restriction ligation, Golden Gate assemblyThe qPCR-based approach presented here should facilitate the development of DNA assembly techniques and

Keywords： Assembly efficiency     DNA assembly     qPCR     Secondary structure     Transformation    

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Design, synthesis and HIV-RT inhibitory activity of novel thiazolidin-4-one derivatives

Hua CHEN, Zaihong GUO, Qingmei YIN, Xiaoxu DUAN, Yunjing GU, Xiaoliu LI

Frontiers of Chemical Science and Engineering 2011, Volume 5, Issue 2,   Pages 231-237 doi: 10.1007/s11705-010-1022-7

Abstract: methyl ( and ) at C-5 on thiazolidin-4-one ring were designed, synthesized and evaluated for their HIV-RTPreliminary anti-HIV-RT test of these derivatives indicated that compounds – , (propionyloxy groupat C-5) showed moderate HIV-RT inhibitory activity and compounds and with methyl at C-5 showed aweak HIV-RT inhibitory activity.activity and that the steric effect might play a critical role in the anti-HIV RT activity.

Keywords： 5-subsituted thiazolidin-4-ones     Pummerer reaction     anti-HIV-RT activity     SARs    

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Bacterial inactivation, DNA damage, and faster ATP degradation induced by ultraviolet disinfection

Chao Yang, Wenjun Sun, Xiuwei Ao

Frontiers of Environmental Science & Engineering 2020, Volume 14, Issue 1, doi: 10.1007/s11783-019-1192-6

Abstract: We used quantitative polymerase chain reaction (qPCR) to investigate DNA damage and evaluated adenosineThe results of qPCR effectively showed the DNA damage induced by UV when using longer gene amplicons,

Keywords： UV disinfection     DNA damage     qPCR     ATP    

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Smartphone-Imaged HIV-1 Reverse-Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) on a Chip Article

Gregory L. Damhorst,Carlos Duarte-Guevara,Weili Chen,Tanmay Ghonge,Brian T. Cunningham,Rashid Bashir

Engineering 2015, Volume 1, Issue 3,   Pages 324-335 doi: 10.15302/J-ENG-2015072

Abstract: In this paper, we perform reverse-transcription loop-mediated isothermal amplification (RT-LAMP) on minimallyOur integrated assay shows amplification from as few as three viruses in a ~ 60 nL RT-LAMP droplet, correspondingThe technology contains greater power in a digital RT-LAMP approach that could be scaled up for the determinationWe demonstrate that all aspects of this viral load approach, from a drop of blood to imaging the RT-LAMP

Keywords： human immunodeficiency virus (HIV)     viral load     loop-mediated isothermal amplification     smartphone     point-of-care    

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Effect of the ultraviolet/chlorine process on microbial community structure, typical pathogens, and antibiotic resistance genes in reclaimed water

Frontiers of Environmental Science & Engineering 2022, Volume 16, Issue 8, doi: 10.1007/s11783-022-1521-z

Abstract:

• UV/chlorine can effectively remove VBNC pathogens, ARGs and MGEs in reclaimed water.

Keywords： UV/chlorine process     Pathogen     Antibiotic resistance genes     High-throughput qPCR     Reclaimed water    

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Design and use of group-specific primers and probes for real-time quantitative PCR

Juntaek LIM, Seung Gu SHIN, Seungyong LEE, Seokhwan HWANG

Frontiers of Environmental Science & Engineering 2011, Volume 5, Issue 1,   Pages 28-39 doi: 10.1007/s11783-011-0302-x

Abstract: Real-time quantitative polymerase chain reaction (qPCR) has gained popularity as a technique to detectAlthough qPCR is a very useful technique for nucleic acid quantification, accurately quantifying theMany aspects of conducting qPCR assays have become increasingly routine and automated; however, one ofWe demonstrate the effectiveness of these guidelines by reviewing the use of qPCR to study anaerobicprocesses and biologic nutrient removal processes. qPCR assays using group-specific primers and probes

Keywords： absolute quantification     design guideline     primer     probe     real-time quantitative polymerase chain reaction (qPCR    

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Analysis of differential expression of genes induced by ethephon in elongating internodes of maize plants

Xiaoyi WEI,Weiqiang ZHANG,Qian ZHANG,Pei SUN,Zhaohu LI,Mingcai ZHANG,Jianmin LI,Liusheng DUAN

Frontiers of Agricultural Science and Engineering 2016, Volume 3, Issue 3,   Pages 263-282 doi: 10.15302/J-FASE-2016103

Abstract: Plant growth regulators (PGRs) are commonly used in cereal cropping systems to restrict plant height and control lodging. Ethephon has been reported to shorten internodes and increase grain yield of maize. To analyze the transcriptomic profiles of maize internode elongation following ethephon treatment, differentially expressed genes were compared between the treatment and control samples of inbred line Zong 31 using the Affymetrix Maize Genome Array. According to the microarray data, 326 probe sets showed significant change in expression. Further research revealed that the most remarkable effects of ethephon on maize internodes elongation occurred during a 48 h period, when 89 differentially expressed genes were detected. There were dramatic change in transcript levels at 24 h and six Auxin transport genes and four gibberellin biosynthesis pathway genes were differentially expressed in Zong 31 in response to ethephon treatment. In summary, we showed that gaseous ethylene release is involved in internode meristem cell elongation through the regulation of plant hormone signaling in maize. This work provides a platform for studies in which candidate genes will be functionally tested for involvement in internode elongation.

Keywords： maize     ethephon     internode elongation     microarray     qPCR    

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Title Author Date Type Operation

Redesigned Duplex RT-qPCR for the Detection of GI and GII Human Noroviruses

Danlei Liu, Zilei Zhang, Qingping Wu, Peng Tian, Haoran Geng, Ting Xu, Dapeng Wang

Journal Article

quantification of 3′-terminal 2′-O-methylated small RNAs by utilizing oxidative deep sequencing and stem-loop RT-qPCR

Journal Article

Developing a Transformation-Independent and Unbiased qPCR Assay to Rapidly Evaluate the Determinants

Xiaoyan Ma, Xinxin Liang, Yi-Xin Huo

Journal Article

Design, synthesis and HIV-RT inhibitory activity of novel thiazolidin-4-one derivatives

Hua CHEN, Zaihong GUO, Qingmei YIN, Xiaoxu DUAN, Yunjing GU, Xiaoliu LI

Journal Article

Bacterial inactivation, DNA damage, and faster ATP degradation induced by ultraviolet disinfection

Chao Yang, Wenjun Sun, Xiuwei Ao

Journal Article

Smartphone-Imaged HIV-1 Reverse-Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) on a Chip

Gregory L. Damhorst,Carlos Duarte-Guevara,Weili Chen,Tanmay Ghonge,Brian T. Cunningham,Rashid Bashir

Journal Article

Effect of the ultraviolet/chlorine process on microbial community structure, typical pathogens, and antibiotic resistance genes in reclaimed water

Journal Article

Design and use of group-specific primers and probes for real-time quantitative PCR

Juntaek LIM, Seung Gu SHIN, Seungyong LEE, Seokhwan HWANG

Journal Article

Analysis of differential expression of genes induced by ethephon in elongating internodes of maize plants

Xiaoyi WEI,Weiqiang ZHANG,Qian ZHANG,Pei SUN,Zhaohu LI,Mingcai ZHANG,Jianmin LI,Liusheng DUAN

Journal Article